Novel DNA sensor for guanine content.
| Data(s) |
01/02/2002
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|---|---|
| Resumo |
The bifunctional complex [Ru(TAP)(2)POQ-Nmet](2+), 1, formed with a [Ru(TAP)(2)Phen](2+) metallic unit linked to a quinoline moiety, and [Ru(TAP)(2)Phen](2+), 2, as reference, have been tested as photoprobes of DNA. Interestingly, 1 exhibits an emission enhancement of a factor of 16-17 upon binding to calf thymus DNA. Moreover, this emission is modulated by the nucleic base content of the polynucleotide. It varies by almost an order of magnitude from a polynucleotide containing 100% of G-C to a guanine-free nucleic acid where the excited-state lifetime reaches about 2 micros. The origins of these interesting properties are analyzed by comparing 1 with reference 2 in the presence of different polynucleotides. Comparative Study Journal Article Research Support, Non-U.S. Gov't info:eu-repo/semantics/published |
| Formato |
No full-text files |
| Identificador |
uri/info:doi/10.1021/ic0108944 uri/info:pii/ic0108944 uri/info:pmid/11849097 local/VX-005287 http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/76669 |
| Idioma(s) |
en |
| Fonte |
Inorganic chemistry, 41 (4 |
| Palavras-Chave | #Chimie #Chimie inorganique #Chimie organique #Chimie de coordination #Photochimie #Animals #Binding Sites #Cattle #DNA -- chemistry #Guanine -- analysis #Hydrogen-Ion Concentration #Models, Molecular #Molecular Structure #Organometallic Compounds -- chemistry #Polynucleotides -- chemistry #Ruthenium -- chemistry #Spectrophotometry #Thymine -- analysis #Time Factors |
| Tipo |
info:eu-repo/semantics/article info:ulb-repo/semantics/articlePeerReview info:ulb-repo/semantics/openurl/article |
