Interstitial insertion of varying amounts of ABL-containing genetic material into chromosome 22 in Ph-negative CML.
Data(s) |
01/04/1990
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Resumo |
We studied the cells from three selected patients with Ph-chromosome-negative chronic myeloid leukemia (CML) by Southern blotting, polymerase chain reaction, and in situ hybridization of informative probes to metaphase chromosomes. All three patients had rearrangement of M-BCR sequences in the BCR gene and expression of one or other of the mRNA species characteristic of Ph-positive CML. Leukemic metaphases studied after trypsin-Giemsa banding were indistinguishable from normal. The ABL probe localized both to chromosome 9 and 22 in each case. A probe containing 3' M-BCR sequences localized only to chromosome 22, and not to chromosome 9 as would be expected in Ph-positive CML. Two new probes that recognize different polymorphic regions distal to the ABL gene on chromosome 9 in normal subjects localized exclusively to chromosome 9 in two patients and to both chromosomes 9 and 22 in one patient. These results show that Ph-negative CML with BCR rearrangement is associated with insertion of a variable quantity of chromosome 9 derived material into chromosome 22q11; there is no evidence for reciprocal translocation of material from chromosome 22 to chromosome 9. Journal Article info:eu-repo/semantics/published |
Formato |
No full-text files |
Identificador |
uri/info:pmid/2164119 http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/59447 |
Idioma(s) |
en |
Fonte |
Leukemia, 4 (4 |
Palavras-Chave | #Sciences bio-médicales et agricoles #Base Sequence #Blotting, Southern #Bone Marrow Cells #Chromosome Banding #Chromosomes, Human, Pair 22 -- physiology #DNA -- genetics #DNA Transposable Elements -- physiology #Gene Rearrangement -- physiology #Humans #Leukemia, Myelogenous, Chronic, BCR-ABL Positive -- genetics #Leukemia, Myeloid, Chronic, Atypical, BCR-ABL Negative -- genetics #Metaphase #Molecular Sequence Data #Nucleic Acid Hybridization #Polymerase Chain Reaction #Proto-Oncogene Proteins -- genetics #Proto-Oncogene Proteins c-abl #Translocation, Genetic -- physiology |
Tipo |
info:eu-repo/semantics/article info:ulb-repo/semantics/articlePeerReview info:ulb-repo/semantics/openurl/article |