Preparation of monolithic silica column with strong cation-exchange stationary phase for capillary electrochromatography
Data(s) |
01/05/2005
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Resumo |
A monolithic silica based strong cation-exchange stationary phase was successfully prepared for capillary electrochromatography. The monolithic silica matrix from a sol-gel process was chemically modified by treatment with 3-mercaptopropyltrimethoxysilane followed by a chemical oxidation procedure to produce the desired function. The strong cation-exchange stationary phase was characterized by its substantial and stable electroosmotic flow (EOF), and it was observed that the EOF value of the prepared column remained almost unchanged at different buffer pH values and slowly decreased with increasing phosphate concentration in the mobile phase. The monolithic silica column with strong cation-exchange stationary phase has been successfully employed in the electrochromatographic separation of beta-blockers and alkaloids extracted from traditional Chinese medicines (TCMs). The column efficiencies for the tested beta-blockers varied from 210,000 to 340,000 plates/m. A peak compression effect was observed for atenolol with the mobile phase having a low phosphate concentration. |
Identificador | |
Idioma(s) |
英语 |
Fonte |
谢传辉;胡继伟;肖华;苏兴业;董靖;田瑞军;何治柯;邹汉法.Preparation of monolithic silica column with strong cation-exchange stationary phase for capillary electrochromatography,Journal of Separation Science,2005,28(8):751-756 |
Palavras-Chave | #electrochromatography #silica monolith #strong cation-exchange #basic pharmaceuticals |
Tipo |
期刊论文 |