Modification of a poly(methyl methacrylate) injection-molded microchip and its use for high performance analysis of DNA
Data(s) |
01/02/2005
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Resumo |
Inexpensive and permanently modified poly(methyl methacrylate)(PMMA) microchips were fabricated by an injection-molding process. A novel sealing method for plastic microchips at room temperature was introduced. Run-to-run and chip-to-chip reproducibility was good, with relative standard deviation values between 1-3% for the run-to-run and less than 2.1% for the chip-to-chip comparisons. Acrylonitrile-butadiene-styrene (ABS) was used as an additive in PMMA substrates. The proportions of PMMA and ABS were optimized. ABS may be considered as a modifier, which obviously improved some characteristics of the microchip, such as the hydrophilicity and the electro-osmotic flow (EOF). The detection limit of Rhodamine 6G dye for the modified microchip on the home-made microchip analyzer showed a dramatic 100-fold improvement over that for the unmodified PMMA chip. A detection limit of the order of 10(-20) mole has been achieved for each injected phiX-174/HaeIII DNA fragment with the baseline separation between 271 and 281 bp, and fast separation of 11 DNA restriction fragments within 180 seconds. Analysis of a PCR product from the tobacco ACT gene was performed on the modified microchip as an application example. |
Identificador | |
Idioma(s) |
英语 |
Fonte |
周小棉; 戴忠鹏; 刘欣; 罗勇; 王辉; 林炳承.Modification of a Poly(methyl methacrylate) Injection-molded Microchip and Its Use for High Performance Analysis of DNA,Journal of Separation Science,2005,28(3):225-233 |
Palavras-Chave | #DNA separation #electrophoretic microchip #fabrication #modification |
Tipo |
期刊论文 |