Purification of High-quality Plastid DNA from the Brown Alga Laminaria japonica Sporophytes


Autoria(s): Fu, Wandong; Yao, Jianting; Wang, Xiuliang; Liu, Fuli; Chong, Zhuo; Duan, Delin
Data(s)

01/06/2008

Resumo

Extracting DNA from a variety of algae is rather difficult because of high levels of polysaccharides, tannins, and phenolics as these interfere with DNA isolation and downstream applications. High-quality plastid DNA (ptDNA) purification is particularly difficult because of its small proportion in total genomic DNA. This report describes an improved protocol for ptDNA purification that efficiently produces high-quality ptDNA from sporophytes of Laminaria japonica and several other algae. This improved protocol simplifies procedures for ptDNA purification and improves yield to 150-200 mu g of ptDNA per 100 g of frozen algal tissue. Polymerase chain reaction (PCR) amplification of conserved sequences has been used to verify purity of the ptDNA product.

Extracting DNA from a variety of algae is rather difficult because of high levels of polysaccharides, tannins, and phenolics as these interfere with DNA isolation and downstream applications. High-quality plastid DNA (ptDNA) purification is particularly difficult because of its small proportion in total genomic DNA. This report describes an improved protocol for ptDNA purification that efficiently produces high-quality ptDNA from sporophytes of Laminaria japonica and several other algae. This improved protocol simplifies procedures for ptDNA purification and improves yield to 150-200 mu g of ptDNA per 100 g of frozen algal tissue. Polymerase chain reaction (PCR) amplification of conserved sequences has been used to verify purity of the ptDNA product.

Identificador

http://ir.qdio.ac.cn/handle/337002/5549

http://www.irgrid.ac.cn/handle/1471x/166377

Idioma(s)

英语

Fonte

Fu, Wandong; Yao, Jianting; Wang, Xiuliang; Liu, Fuli; Chong, Zhuo; Duan, Delin.Purification of High-quality Plastid DNA from the Brown Alga Laminaria japonica Sporophytes,PLANT MOLECULAR BIOLOGY REPORTER,2008,26(2):114-120

Palavras-Chave #Biochemical Research Methods; Plant Sciences #Brown alga #Laminaria japonica #Plastid DNA #Ultracentrifugation
Tipo

期刊论文