Spectroscopic properties of the C-phycocyanin-allophycocyanin conjugate and the isolated phycobilisomes from Spirulina platensis


Autoria(s): Wang, GC; Zhou, BC; Tseng, CK
Data(s)

1997

Resumo

C-phycocyanin (CPC) and allophycocyanin (APC) were purified from Spirulina platensis, then the CPC was attached covalently to the APC by reacting their epsilon-amino groups. The excitation energy could be transferred from the CPC to the APC in the CPC-APC conjugate. Intact phycobilisomes (PBS), consisting of CPC, APC, colourless linker polypeptides, and APC B or L-cm, were isolated from S. platensis. Spectroscopic properties of the isolated PBSs kept at 20 degrees C for various times showed that the connection between the APC and the APC B or L-cm was looser than that between the CPC and the APC in the isolated PBSs. The CPC-APC conjugate was more stable than the isolated PBSs, and the linker polypeptides had a minor influence on the excitation energy transfer characteristic between different phycobiliproteins in the PBS.

Identificador

http://ir.qdio.ac.cn/handle/0/3383

http://www.irgrid.ac.cn/handle/1471x/166346

Fonte

Wang, GC; Zhou, BC; Tseng, CK.Spectroscopic properties of the C-phycocyanin-allophycocyanin conjugate and the isolated phycobilisomes from Spirulina platensis,PHOTOSYNTHETICA,1997,34(1):57-65

Palavras-Chave #Plant Sciences #ENERGY-TRANSFER
Tipo

期刊论文