Isolation and characterization of microsatellites in yellow perch (Perca flavescens)


Autoria(s): Li, L.; Wang, H. P.; Givens, C.; Czesny, S.; Brown, B.
Data(s)

01/07/2007

Resumo

A total of 45 microsatellite loci from yellow perch, Perca flavescens, were isolated and characterized. Among the 45 microsatellite loci, 32 had more than two alleles. A wild population of P. flavescens (n = 48) was used to examine the allele range of the microsatellite loci. Mendelian inheritance of alleles was confirmed by examining the amplified products in pair-mated families. The number of alleles for the 32 polymorphic loci varied from two to 16, and observed heterozygosity ranged between 0.024 (YP79) and 0.979 (YP60). Cross-species polymorphic amplification in four other Percidae species was successful for 22 loci.

A total of 45 microsatellite loci from yellow perch, Perca flavescens, were isolated and characterized. Among the 45 microsatellite loci, 32 had more than two alleles. A wild population of P. flavescens (n = 48) was used to examine the allele range of the microsatellite loci. Mendelian inheritance of alleles was confirmed by examining the amplified products in pair-mated families. The number of alleles for the 32 polymorphic loci varied from two to 16, and observed heterozygosity ranged between 0.024 (YP79) and 0.979 (YP60). Cross-species polymorphic amplification in four other Percidae species was successful for 22 loci.

Identificador

http://ir.qdio.ac.cn/handle/337002/1580

http://www.irgrid.ac.cn/handle/1471x/166006

Idioma(s)

英语

Fonte

Li, L.; Wang, H. P.; Givens, C.; Czesny, S.; Brown, B..Isolation and characterization of microsatellites in yellow perch (Perca flavescens),MOLECULAR ECOLOGY NOTES,2007,7(4):600-603

Palavras-Chave #Biochemistry & Molecular Biology; Ecology; Evolutionary Biology #microsatellite #Perca flavescens #tetranucleotide #trinucleotide #yellow perch
Tipo

期刊论文