The involvement of HSP22 from bay scallop Argopecten irradians in response to heavy metal stress


Autoria(s): Zhang, Lei; Wang, Lingling; Song, Linsheng; Zhao, Jianmin; Qiu, Limei; Dong, Chaohua; Li, Fengmei; Zhang, Huan; Yang, Guanpin
Data(s)

01/04/2010

Resumo

Heat shock protein 22 (HSP22) is an important member of small heat shock protein (sHSP) subfamily which plays a key role in the process of protecting cells, facilitating the folding of nascent peptides, and responding to stress. In the present study, the cDNA of HSP22 was cloned from Argopecten irradians (designated as AiHSP22) by rapid amplification cDNA end (RACE) based on the expressed sequence tags (ESTs). The full-length cDNA of AiHSP22 was of 1,112 bp, with an open reading frame of 588 bp encoding a polypeptide of 195 amino acids. The deduced amino acid sequence of AiHSP22 showed high similarity to previously identified HSP22s. The expression patterns of AiHSP22 mRNA in different tissues and in haemocytes of scallops exposed to Cd2+, Pb2+ or Cu2+ were investigated by real-time quantitative RT-PCR. The mRNA of AiHSP22 was constitutively expressed in all examined tissues, including haemocyte, muscle, kidney, gonad, gill and heart. The expression level in heart and muscle was higher than that in other tissues. The mRNA level of AiHSP22 in haemocytes was up-regulated after a 10 days exposure of scallops to Cu2+, Pb2+ and Cd2+. However, the expression of AiHSP22 did not increase linearly along with the rise of heavy metal concentration. Different concentrations of the same metal resulted in different effects on AiHSP22 expression. The sensitive response of AiHSP22 to Cu2+, Pb2+ and Cd2+ stress indicated that it could be developed as an indicator of exposure to heavy metals for the pollution monitoring programs in aquatic environment.

Heat shock protein 22 (HSP22) is an important member of small heat shock protein (sHSP) subfamily which plays a key role in the process of protecting cells, facilitating the folding of nascent peptides, and responding to stress. In the present study, the cDNA of HSP22 was cloned from Argopecten irradians (designated as AiHSP22) by rapid amplification cDNA end (RACE) based on the expressed sequence tags (ESTs). The full-length cDNA of AiHSP22 was of 1,112 bp, with an open reading frame of 588 bp encoding a polypeptide of 195 amino acids. The deduced amino acid sequence of AiHSP22 showed high similarity to previously identified HSP22s. The expression patterns of AiHSP22 mRNA in different tissues and in haemocytes of scallops exposed to Cd(2+), Pb(2+) or Cu(2+) were investigated by real-time quantitative RT-PCR. The mRNA of AiHSP22 was constitutively expressed in all examined tissues, including haemocyte, muscle, kidney, gonad, gill and heart. The expression level in heart and muscle was higher than that in other tissues. The mRNA level of AiHSP22 in haemocytes was up-regulated after a 10 days exposure of scallops to Cu(2+), Pb(2+) and Cd(2+). However, the expression of AiHSP22 did not increase linearly along with the rise of heavy metal concentration. Different concentrations of the same metal resulted in different effects on AiHSP22 expression. The sensitive response of AiHSP22 to Cu(2+), Pb(2+) and Cd(2+) stress indicated that it could be developed as an indicator of exposure to heavy metals for the pollution monitoring programs in aquatic environment.

Chinese Ministry of Science and Technology [2006AA10A402]; Chinese Ministry of Agriculture [nyhyzx07-047]; NSFC [30730070]

Identificador

http://ir.qdio.ac.cn/handle/337002/146

http://www.irgrid.ac.cn/handle/1471x/166001

Idioma(s)

英语

Publicador

SPRINGER

Fonte

Zhang, Lei; Wang, Lingling; Song, Linsheng; Zhao, Jianmin; Qiu, Limei; Dong, Chaohua; Li, Fengmei; Zhang, Huan; Yang, Guanpin.The involvement of HSP22 from bay scallop Argopecten irradians in response to heavy metal stress,MOLECULAR BIOLOGY REPORTS,2010,37(4):1763-1771

Palavras-Chave #Biochemistry & Molecular Biology #HSP22 #Argopecten irradians #Gene cloning #Biomarker #Heavy metals
Tipo

期刊论文