Interaction of brilliant cresyl blue and methylene green with DNA studied by spectrophotometric and voltammetric methods
Data(s) |
1996
|
---|---|
Resumo |
The binding behavior of two cationic dyes, brilliant cresyl blue (BCB) and methylene green (MG) to calf thymus DNA was studied by spectrophotometric and voltammetric methods. A red shift of the adsorption spectra and hypochromism accompany the binding of BCB and MG to calf thymus DNA. In 5 x 10(-2) mol dm(-3) NaCl, 5 x 10(-3) mol dm(-3) tris-HCl pH 6.87 buffer solution, the apparent binding constants are: K-BCB+ 3.0 x 10(4)M(-1) (N = 4.13) and K-MG+ = 8.8 x 10(4)M(-1) (n = 4.44). Electrochemical studies show that the formal potentials shift negatively upon addition of DNA, indicating that the oxidized forms of the dyes have stronger affinity to DNA than the reduced ones. K-BCB+/K-BCBH and K-MG+/K-MGH are evaluated to be 10.39 and 7.04. respectively. Our investigation suggests that the two cationic dyes interact with DNA predominantly via electrostatic interaction. |
Identificador | |
Idioma(s) |
英语 |
Fonte |
Liu JL;Li JH;Dong SJ.Interaction of brilliant cresyl blue and methylene green with DNA studied by spectrophotometric and voltammetric methods,ELECTROANALYSIS,1996,8(8-9):803-807 |
Palavras-Chave | #BINDING #COMPLEXES #FLUORESCENCE #OXIDATION #ETHIDIUM |
Tipo |
期刊论文 |