Amperometric biosensor for tyrosinase inhibitors in a pure organic phase
Data(s) |
1996
|
---|---|
Resumo |
The determination of benzoic acid, thiourea and 2-mercaptoethanol in three pure organic solvents, viz., chloroform, chlorobenzene and 1,2-dichlorobenzene, by using an amperometric cryohydrogel tyrosinase biosensor is described. Measurements were carried out with phenol as the enzyme substrate. Kinetic parameters (K-i and I-50) were determined in the three solvents for various inhibitors. The sensor showed the most sensitive measurements to these inhibitors in pure chloroform. The solvent-induced deviation of the biosensor to thiourea was evaluated by means of Hill coefficients. The smallest deviation as observed in 1,2-dichlorobenzene, owing to the high hydrophobicity of this solvent. The nature of the inhibition process and its reversibility mere also examined. |
Identificador | |
Idioma(s) |
英语 |
Fonte |
Deng Q;Dong SJ .Amperometric biosensor for tyrosinase inhibitors in a pure organic phase,ANALYST,1996,121(12):1979-1982 |
Palavras-Chave | #NONAQUEOUS SOLVENTS #ELECTRODE |
Tipo |
期刊论文 |