Sol-gel thin-film immobilized soybean peroxidase biosensor for the amperometric determination of hydrogen peroxide in acid medium


Autoria(s): Wang BQ; Li B; Wang ZX; Xu GB; Wang Q; Dong SJ
Data(s)

1999

Resumo

An acid-stable soybean-peroxidase biosensor was devel oped by immobilizing the enzyme in a sol-gel thin film. Methylene blue was used as a mediator because of its high electron-transfer efficiency. The sol-gel thin film and enzyme membrane were characterized by FT-IR, and the effects of pH, operating potential, and temperature were explored for optimum analytical performance by using the amperometric method. The H2O2 sensor exhibited a fast response (5 s), high sensitivity (27.5 mu A/mM), as well as good thermostability and long-term stability. In addition, the performance of the biosensor was investigated using flow-injection analysis (FIA).

Identificador

http://202.98.16.49/handle/322003/21879

http://www.irgrid.ac.cn/handle/1471x/155674

Idioma(s)

英语

Fonte

Wang BQ;Li B;Wang ZX;Xu GB;Wang Q;Dong SJ.Sol-gel thin-film immobilized soybean peroxidase biosensor for the amperometric determination of hydrogen peroxide in acid medium,ANALYTICAL CHEMISTRY,1999,71(10):1935-1939

Palavras-Chave #REDUCED NICOTINAMIDE COENZYMES #MODIFIED ELECTRODES #HORSERADISH-PEROXIDASE #ELECTROCATALYTIC OXIDATION #ENZYME ELECTRODES #METHYLENE GREEN #GLUCOSE-OXIDASE #GLASS #COMPOSITE #ENCAPSULATION
Tipo

期刊论文