Chemiluminescent determination of luminol and hydrogen peroxide using hematin immobilized in the bulk of a carbon paste electrode


Autoria(s): Xu GB; Dong SJ
Data(s)

1999

Resumo

A new method for immobilization of a chemiluminescent reagent is presented. It is based on immobilizing hematin, a catalyst for luminol reaction, in the bulk of a carbon paste electrode. Bulk-immobilization allows renewal of the surface by simple polishing or cutting to expose anew and fully active surface in the case of fouling or deactivation by other means. By using a hematin-modified carbon paste electrode, the applied potential shifted negatively compared with that of unmodified carbon paste electrode or a glassy carbon electrode. The shift in potential changed the reaction processes and effectively stabilized the chemiluminescent signal during successive measurements. Under this condition, the signal was stable during 3 hours of continuous operation. The log-log plots of the emitted light intensity vs. luminol concentration and hydrogen peroxide concentration were linear over the region 10(-8)-10(-3) mol L-1 with a correlation coefficient of 0.999 and 3.9 x 10(-6)-10(-3) mol L-1 with a correlation coefficient of 0.994, respectively. Application of this method for other chemiluminescent and bioluminescent systems is suggested.

Identificador

http://202.98.16.49/handle/322003/21523

http://www.irgrid.ac.cn/handle/1471x/155496

Idioma(s)

英语

Fonte

Xu GB;Dong SJ.Chemiluminescent determination of luminol and hydrogen peroxide using hematin immobilized in the bulk of a carbon paste electrode,ELECTROANALYSIS,1999,11(16):1180-1184

Palavras-Chave #PERFORMANCE LIQUID-CHROMATOGRAPHY #CHEMI-LUMINESCENCE #FIBER MICROELECTRODES #LIPID HYDROPEROXIDES #FLOW #ENZYMES #ARCHITECTURE #IMMUNOASSAY #SEPARATION #COPPER
Tipo

期刊论文