Interactions of Safranin T with nucleic acids
Data(s) |
2001
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Resumo |
The interactions of Safranin T (ST) with several nucleic acids have been investigated by electrochemical, UV-visible and CD spectroscopic techniques. The form of the nucleic acid-ST complexes is sensitive to the ratio of the two species. Two electrochemically inactive complexes such as, nucleic acid-ST and nucleic acid-2ST, were formed while ST interacts with nucleic acids. Two processes were obtained from spectral experiments: (1) at the high value of R (R is defined as the ratio of the total concentration of ST to that of nucleic acid), ST is groove-binding with stacking, (2) st the low value of R, ST is groove-binding without stacking. Intrinsic binding constants were obtained by spectral methods. The experiments also show that electrostatic binding plays an important role in the interaction of ST with nucleic acids. |
Identificador | |
Idioma(s) |
英语 |
Fonte |
Wang ZX;Liu DJ;Dong SJ .Interactions of Safranin T with nucleic acids,CHINESE JOURNAL OF CHEMISTRY,2001,19(7):662-669 |
Palavras-Chave | #CALF THYMUS DNA #CATIONIC PORPHYRIN LIGANDS #CONFORMATIONAL TRANSITION #NONINTERCALATIVE BINDING #CD |
Tipo |
期刊论文 |