Amperometric biosensor for hydrogen peroxide based on sol-gel/hydrogel composite thin film


Autoria(s): Chen X; Wang BQ; Dong SJ
Data(s)

2001

Resumo

A reagentless amperometric hydrogen peroxide biosensor was developed. Horseradish peroxidase (HRP) was immobilized in a novel sol-gel organic-inorganic hybrid matrix that is composed of silica sol and a grafting copolymer of poly(vinyl alcohol) with 4-vinylpyridine (PVA-g-PVP). Tetrathiafulvalene (TTF) was employed as a mediator and could lower the operating potential to -50 mV (versus Ag/AgCl). The sensor achieved 95% of the steady-state current in 15 s. Linear calibration for hydrogen peroxide was up to 1.3 mM with the detection limit of 2.5 x 10(-7)M. The enzyme electrode retained about 94% of its initial activity after 30 days of storage in a dry state at 4 degreesC.

Identificador

http://202.98.16.49/handle/322003/20543

http://www.irgrid.ac.cn/handle/1471x/155006

Idioma(s)

英语

Fonte

Chen X;Wang BQ;Dong SJ.Amperometric biosensor for hydrogen peroxide based on sol-gel/hydrogel composite thin film,ELECTROANALYSIS,2001,13(14):1149-1152

Palavras-Chave #HORSERADISH-PEROXIDASE #ENZYME ELECTRODE #GLUCOSE-OXIDASE #GEL MATERIALS #CARBON #SENSOR #IMMOBILIZATION #ENCAPSULATION #MATRICES #SILICATE
Tipo

期刊论文