Detection of anti-human serum albumin antibody using surface plasmon resonance biosensor
Data(s) |
2000
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Resumo |
The biosensor based on surface plasmon resonance(SPR) technology is a very useful tool to study the interaction between biomolecles. The main advantages of this technique is to "visualize" macromolecular interactions directly in real time, and in a label-free mode rather than indirect methods like enzyme-linked immunosorbent assays (ELISAs). We immobilize human serum albumin (HSA) to the carboxymethyldextran-modified sensor chip surface covalently to detect the activity of anti-HSA in serum, and regenerate the surface with .1 mol/L phosphoric acid. The results show that SPR biosensor can detect the activity of anti-HSA in real-time quickly and the sensor chip can be used over 100 cycles. |
Identificador | |
Idioma(s) |
中文 |
Fonte |
Cui XQ;Pei RJ;Yang XR.Detection of anti-human serum albumin antibody using surface plasmon resonance biosensor,CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2000,28(8):950-955 |
Palavras-Chave | #SENSORS #IMMOBILIZATION #LIGANDS |
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期刊论文 |