Silica sol-gel composite film as an encapsulation matrix for the construction of an amperometric tyrosinase-based biosensor


Autoria(s): Wang B; Zhang J; Dong S
Data(s)

2000

Resumo

An amperometric tyrosinase enzyme electrode for the determination of phenols was developed by a simple and effective immobilization method using sol-gel techniques. A grafting copolymer was introduced into sol-gel solution and the composition of the resultant organic-inorganic composite material was optimized, the tyrosinase retained its activity in the sol-gel thin film and its response to several phenol compounds was determined at 0 mV vs. Ag/AgCl (sat. KCI). The dependences of the current response on pH, oxygen level and temperature were studied, and the stability of the biosensor was also evaluated. The sensitivity of the biosensor for catechol, phenol and p-cresol was 59.6, 23.1 and 39.4 muA/mM, respectively. The enzyme electrode maintained 73% of its original activity after intermittent use for three weeks when storing in a dry state at 4 degreesC. (C) 2000 Elsevier Science S.A. All rights reserved.

Identificador

http://202.98.16.49/handle/322003/19499

http://www.irgrid.ac.cn/handle/1471x/154484

Idioma(s)

英语

Fonte

Wang B;Zhang J;Dong S.Silica sol-gel composite film as an encapsulation matrix for the construction of an amperometric tyrosinase-based biosensor,BIOSENSORS & BIOELECTRONICS,2000,15(7-8):397-402

Palavras-Chave #POLY(VINYL ALCOHOL) #ENZYME ELECTRODE #PHENOLS #COPOLYMER #PHASE
Tipo

期刊论文