Europium ion labeling anti-HBs
| Data(s) |
2000
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|---|---|
| Resumo |
A microsecond time-resolved laser fluorescence spectroscopic analysis set was developed, A chelate-cyclic anhydride of diethylenetrimin pentaacetic acid anhydride (DTPAA) was synthesized. An anti-HBs antibody was purified, A EU3+ -DTPAA-anti-HBs label was prepared by two step procedure. We described the optimal condtion with EU3+ as marker and DTPAA as chelate bounding to antibody molecule. Labeling parameters such as solvent pH, protein and chelate molar ratio, reaction time, separation method were discussed in detail. |
| Identificador | |
| Idioma(s) |
中文 |
| Fonte |
Pan LH;Ma SY;Zhao WJ;Lin M;Luan YC;Yun Q.Europium ion labeling anti-HBs,CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2000,28(11):1439-1442 |
| Tipo |
期刊论文 |
