Preparation and properties of abzyme with thyroxine deiodinase
Data(s) |
2002
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Resumo |
The abzyme (Se-6E8) with a higher thyroxine deiodinase activity was prepared by modifying the serine residues of monoclonal antibody (6E8)with phenylmethanesulfonyl fluoride and sodium hydrogen selenide, and the 6E8 against O-methyl-T-4, which is a kind of thyroxine derivatives and was taken as a hapten for the first time. Two bands were found corresponding to the 5.5 kD heavy chain and the 2.7 kD light chain respectively by SDS-PAGE. The characteristics of dissociation constants, pH, and temperature were also studied. The results show that the activity of Se-6E8 is 2 010 U/mumol protein, and the proper temperature and pH of the catalytic reactions is 57 degreesC and 8.2 respectively. |
Identificador | |
Idioma(s) |
中文 |
Fonte |
Sun LW;Liu LY;Zhao DQ.Preparation and properties of abzyme with thyroxine deiodinase,CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2002,23(12):2217-2220 |
Palavras-Chave | #I IODOTHYRONINE DEIODINASE #5'-DEIODINASE #ANTIBODY #ENZYME |
Tipo |
期刊论文 |