Characterization of aconitine-type alkaloids in the flowers of Aconitum kusnezoffii by electrospray ionization tandem mass spectrometry


Autoria(s): Wang Y; Song FR; Xu QX; Liu ZQ; Liu SY
Data(s)

2003

Resumo

The fragmentation mechanism of aconitine-type alkaloids in the flowers of Aconitum kusnezoffii (FAK) was investigated using electrospray ionization tandem mass spectrometry (ESI-MSn) firstly. The analysis of the collision-induced dissociation (CID) spectra of three purified aconitine standards and six previously reported aconitines indicated that the fragmentation of the protonated aconitines at low-energy CID follows a similar pathway. The elimination of a C-8-substituent such as an acetic acid or a fatty acid is the dominant fragmentation mode in MS2. Successive losses of CH3COOH, CH3OH, H2O, BzOH, and CO are the main fragmentation pathways of aconitine-type alkaloids in MS3 spectra. Based on these features, a rapid method for the direct detection and characterization of alkaloids from an ethanolic extract of FAK is described. All the known aconitum alkaloids are detected and a series of lipo-aconitines has been found for the first time in this plant.

Identificador

http://ir.ciac.jl.cn/handle/322003/17727

http://www.irgrid.ac.cn/handle/1471x/153256

Idioma(s)

英语

Fonte

Wang Y;Song FR;Xu QX;Liu ZQ;Liu SY.Characterization of aconitine-type alkaloids in the flowers of Aconitum kusnezoffii by electrospray ionization tandem mass spectrometry,JOURNAL OF MASS SPECTROMETRY,2003,38(9):962-970

Palavras-Chave #NORDITERPENOID ALKALOIDS #ROOTS #MESACONITINE
Tipo

期刊论文