Differential proteomic analysis of neonatal cardiomyocytes in response to beta-adrenergic receptor stimulation
Data(s) |
2006
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Resumo |
beta-Adrenoceptors(beta-ARs) play a critical role in regulating cardiac functions under both physiological and pathological conditions. To further explore the mechanisms through which beta-ARs perform its actions, proteomic approaches were adopted to study the global protein patterns in cultured neonatal rat cardiomyocytes exposed to isoproterenol (ISO). A modified method, "Mirror Images in One Gel", was used to improve the reproducibility and resolution power of two-dimensional electrophoresis. A 2-DE map with a good reproducibility was obtained in which 1281 70 spots were detected and about 1191 +/- 54 spots were matched, with an average matching rate of 92.9%. Nine proteins with significant changes were identified by using peptide mass fingerprinting(PMF) data obtained via MALDI-MS. |
Identificador | |
Idioma(s) |
英语 |
Fonte |
Li ZJ;Liu ZQ;Liu SY;Han QD;Zhang YY.Differential proteomic analysis of neonatal cardiomyocytes in response to beta-adrenergic receptor stimulation,CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2006,22(3):319-323 |
Palavras-Chave | #MASS-SPECTROMETRY #PROTEINS #IDENTIFICATION #GENES |
Tipo |
期刊论文 |