Carbon nanotubes selective destabilization of duplex and triplex DNA and inducing B-A transition in solution
Data(s) |
2006
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Resumo |
Single-walled carbon nanotubes (SWNTs) have been considered as the leading candidate for nano-device applications ranging from gene therapy and novel drug delivery to membrane separations. The miniaturization of DNA-nanotube devices for biological applications requires fully understanding DNA-nanotube interaction mechanism. We report here, for the first time, that DNA destabilization and conformational transition induced by SWNTs are sequence-dependent. Contrasting changes for SWNTs binding to poly[dGdC]:poly[dGdC] and poly[dAdT]:poly[dAdT] were observed. For GC homopolymer, DNA melting temperature was decreased 40 degrees C by SWNTs but no change for AT-DNA. SWNTs can induce B-A transition for GC-DNA but AT-DNA resisted the transition. Our circular dichroism, competitive binding assay and triplex destabilization studies provide direct evidence that SWNTs induce DNA B-A transition in solution and they bind to the DNA major groove with GC preference. |
Identificador | |
Idioma(s) |
英语 |
Fonte |
Li X;Peng YH;Qu XG.Carbon nanotubes selective destabilization of duplex and triplex DNA and inducing B-A transition in solution,NUCLEIC ACIDS RESEARCH,2006,34(13):3670-3676 |
Palavras-Chave | #FLOW LINEAR DICHROISM #CIRCULAR-DICHROISM #BINDING #SEQUENCE #CONDENSATION #POLYMORPHISM #RECOGNITION #NEOMYCIN #AGENTS #FORM |
Tipo |
期刊论文 |