Structural elucidation and identification of alkaloids in Rhizoma Coptidis by electrospray ionization tandem mass spectrometry


Autoria(s): Wang DW; Liu ZQ; Guo MQ; Liu SY
Data(s)

2004

Resumo

Simple, convenient, sensitive and accurate analytical methods are needed for the structural characterization and identification of alkaloid components in Rhizoma Coptidis in traditional Chinese herbal medicine, which has important bioactivity. In this work, the identification of alkaloid compounds in Rhizoma Coptidis was investigated by obtaining molecular mass information using electrospray ionization mass spectrometry (ESI-MS). Multi-stage tandem mass spectrometric (ESI-MSn) data for the alkaloid compounds were used for detailed structural characterization, then structure information was obtained by comparison of the fragmentation mechanisms of both alkaloids in Rhizoma Coptidis and standard samples of berberine, palmatine, coptisine and jatrorrhizine by MS. Based on the results obtained, the structure of a novel compound was elucidated. The results of the experiments demonstrate that ESI-MSn is a sensitive, selective and effective tool for the rapid determination of alkaloids in Rhizoma Coptidis.

Identificador

http://ir.ciac.jl.cn/handle/322003/15453

http://www.irgrid.ac.cn/handle/1471x/151191

Idioma(s)

英语

Fonte

Wang DW;Liu ZQ;Guo MQ;Liu SY.Structural elucidation and identification of alkaloids in Rhizoma Coptidis by electrospray ionization tandem mass spectrometry,JOURNAL OF MASS SPECTROMETRY,2004,39(11):1356-1365

Palavras-Chave #PERFORMANCE LIQUID-CHROMATOGRAPHY #CRUDE DRUGS #CAPILLARY-ELECTROPHORESIS #QUATERNARY ALKALOIDS #MICROSCOPIC ANALYSIS #PHELLODENDRI CORTEX #ION-TRAP #BERBERINE #CONSTITUENTS #PALMATINE
Tipo

期刊论文