Atomic force microscope investigation of large-circle DNA molecules


Autoria(s): Wu AG; Yu LH; Li ZA; Yang HM; Wang EK
Data(s)

2004

Resumo

A circular bacterial artificial chromosome of 148.9 kbp on human chromosome 3 has been extended and fixed on bare mica substrates using a developed fluid capillary flow method in evaporating liquid drops. Extended circular DNA molecules were imaged with an atomic force microscope (AFM) under ambient conditions. The measured total lengths of the whole DNA molecules were in agreement with sequencing analysis data with an error range of +/-3.6%. This work is important groundwork for probing single nucleotide polymorphisms in the human genome, mapping genomic DNA, manipulating biomolecular nanotechnology, and studying the interaction of DNA-protein complexes investigated by AFM.

Identificador

http://ir.ciac.jl.cn/handle/322003/14825

http://www.irgrid.ac.cn/handle/1471x/150574

Idioma(s)

英语

Fonte

Wu AG;Yu LH;Li ZA;Yang HM;Wang EK.Atomic force microscope investigation of large-circle DNA molecules,ANALYTICAL BIOCHEMISTRY,2004,325(2):293-300

Palavras-Chave #CARBON NANOTUBE PROBES #HIGH-RESOLUTION #PLASMID DNA #SUPERCOILED DNA #LONG DNA #SURFACES #MICA #POLYMERASE #DEPOSITION #FLOW
Tipo

期刊论文