Molecular "Wiring" glucose oxidase in supramolecular architecture
Data(s) |
2007
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Resumo |
Supramolecular organized multilayers were constructed by multiwalled carbon nanotubes modified with ferrocene-derivatized poly(allylamine) redox polymer and glucose oxidase by electrostatic self-assembly. From the analysis of voltammetric signals and fluorescence results, a linear increment of the coverage of enzyme per bilayer was estimated, which demonstrated that the multilayer is constructed in a spatially ordered manner. The cyclic voltammograms obtained from the indium tin oxide (ITO) electrodes coated by the (Fc-PAH@CNT/GOx)(n) multilayers revealed that bioelectrocatalytic response is directly correlated to the number of deposited bilayers; that is, the sensitivity is tunable by controlling the number of bilayers associated with ITO electrodes. The incorporation of redox-polymer-functionalized carbon nanotubes (CNT) into enzyme films resulted in a 6-10-fold increase in the glucose electrocatalytic current; the bimolecular rate constant of FADH(2) oxidation (wiring efficiency) was increased up to 12-fold. Impedance spectroscopy data have yielded the electron diffusion coefficient (D-e) of this nanostructure to be over 10(-8) cm(2) s(-1), which is typically higher than those systems without CNT by at least a factor of 10, indicating that electron transport in the new supramolecular architecture was enhanced by communication of the redox active site of enzyme, redox polymer, and CNT. |
Identificador | |
Idioma(s) |
英语 |
Fonte |
Deng L;Liu Y;Yang GC;Shang L;Wen D;Wang F;Xu Z;Dong SJ.Molecular "Wiring" glucose oxidase in supramolecular architecture,BIOMACROMOLECULES ,2007 ,8(7):2063-2071 |
Palavras-Chave | #MULTIWALL CARBON NANOTUBES #FLAVIN-ADENINE-DINUCLEOTIDE #PEAPOD MODIFIED ELECTRODES #REDOX CENTERS #ELECTRICAL COMMUNICATION #ENZYME ELECTRODES #THIN-FILMS #FERROCENE #COMPOSITES #APOENZYME |
Tipo |
期刊论文 |