Functional gold nanoparticles for studying the interaction of lectin with glycosyl complex on living cellular surfaces


Autoria(s): Wang JN; Duan TC; Sun LL; Liu DJ; Wang ZX
Data(s)

2009

Resumo

In this study. lectin-conjugated gold nanoparticles (GNPs) were prepared by standard biotin-streptavidin chemistry. The lectin-conjugated GNPs call be used as ail indicator for studying the interaction of lectin with glycosyl complex on living cellular Surfaces due to the high affinity of the lectin with saccharides. The interactions of two well-known lectins (Ricinus communis agglutinin and concanavalin A) and three different cell lines (HeLa, 293, and 293T) were selected here to establish this assay. Highly binding affinity of R. communis agglutinin with cells was demonstrated by conventional microscopic and UV-visible spectroscopic Studies. In addition, the binding process can be inhibited by galactose, giving further proof of the binding mechanism. (c) 2009 Elsevier Inc. All rights reserved.

Identificador

http://202.98.16.49/handle/322003/12635

http://www.irgrid.ac.cn/handle/1471x/148730

Idioma(s)

英语

Fonte

Wang JN;Duan TC;Sun LL;Liu DJ;Wang ZX.Functional gold nanoparticles for studying the interaction of lectin with glycosyl complex on living cellular surfaces,ANALYTICAL BIOCHEMISTRY,2009,392(1):77-82

Palavras-Chave #PROTEIN INTERACTIONS #CONCANAVALIN-A #RECOGNITION #LIGANDS #CANCER #TOOLS #SIZE
Tipo

期刊论文