Studies on alkaloids binding to GC-rich human survivin promoter DNA using positive and negative ion electrospray ionization mass spectrometry
Data(s) |
2008
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Resumo |
Electrospray ionization mass spectrometry (ESI-MS) was used to investigate the binding of 13 alkaloids to two GC-rich DNA duplexes which are critical sequences in human survivin promoter. Negative ion ESI-MS was first applied to screen the binding of the alkaloids to the duplexes. Six alkaloids (including berberine, jatrorrhizine, palmatine, reserpine, berbamine, and tetrandrine) show complexation with the target DNA sequences. Relative binding affinities were estimated from the negative ion ESI data, and the alkaloids show a binding preference to the duplex with higher GC content. Positive ion ESI mass spectra of the complexes were also recorded and compared with those obtained in negative ion mode. |
Identificador | |
Idioma(s) |
英语 |
Fonte |
Wang ZF;Guo XH;Liu ZQ;Cui MR;Song FR;Liu SY.Studies on alkaloids binding to GC-rich human survivin promoter DNA using positive and negative ion electrospray ionization mass spectrometry,JOURNAL OF MASS SPECTROMETRY,2008,43(3):327-335 |
Palavras-Chave | #DOUBLE-STRANDED OLIGODEOXYNUCLEOTIDES #CYTOTOXIC PROTOBERBERINE ALKALOIDS #NONCOVALENT COMPLEXES #DUPLEX DNA #BERBERINE DIMERS #TOPOISOMERASE-I #DRUG DISCOVERY #ESI-MS #AFFINITIES #SELECTIVITY |
Tipo |
期刊论文 |