Applications of homemade kit in mutation detection of genes


Autoria(s): Zhao, CX; Xu, GW; Shi, XZ; Ma, JM; Zhang, Y; Lu, S; Yang, Q
Data(s)

01/04/2004

Resumo

Several methods of mutation detection, such as single-strand conformation polymorphism (SSCP), tandem SSCP/heteroduplex analysis and SNaPshot analysis were developed using homemade kit on ABI 310 genetic analyzer, and were successfully applied to mutation detection of 31 colorectal tumor samples. The sieving capability of homemade kit and commercial kit were compared, results demonstrate that homemade kit has higher resolution and shorter analysis time. In clinical tumor samples, 26% K-ras (exon 1) and 24% p53 (exons 7-8) were found to have mutations, and all mutations were single point variations. A majority of mutations occurred in one gene, only 1 tumor contained alterations in the two genes, which indicates that development of colorectal cancer lies on alternate pathways, and may correlate with different gene mutations.

Identificador

http://159.226.238.44/handle/321008/81587

http://www.irgrid.ac.cn/handle/1471x/136638

Idioma(s)

英语

Fonte

赵春霞;许国旺;石先哲;马坚妹;张岩;吕申;杨青.Applications of homemade kit in mutation detection of genes,Science in China Series B Chemistry (中国科学B),2004,47(2):166-172

Palavras-Chave #single nucleotide polymorphism (SNP) #single-strand conformation pollymorphism (SSCP) #heteroduplex analysis (HA) #SNaPshot #linear polyacrylamide (LPA) #polydimethylacrylamide (PDMA)
Tipo

期刊论文