Inhibiting Survivin Expression Increases The Radiosensitivity of Human Hepatoma HepG2 Cells to High-LET Radiation
Data(s) |
2010
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Resumo |
The influence of survivin expression on the radiosensitivity of tumor cells to high linear energy transfer (LET) radiation is investigated. Survivin-specific short-interfering RNA (siRNA) oligonucleotides were synthesized based on the survivin sequence provided by GenBank. Human hepatoma HepG2 cells were transfected with survivin-specific siRNA to inhibit its expressions. It was found that the transfection with surviving-specific siRNA increased the levels of G2/M arrest and the apoptotic rates induced by radiation in HepG2 cells. After exposure to high-LET carbon ions, a reduced clonogenic survival effect was observed in the cells treated with siRNA. These results show that survivin plays a key role in mediating the radioresistance of cells to high-LET radiation. |
Identificador | |
Idioma(s) |
英语 |
Fonte |
Jin, XD; Li, Q; Li, P; Wu, QF; Tao, JJ; Hao, JF; Dai, ZY; Liu, XG.Inhibiting Survivin Expression Increases The Radiosensitivity of Human Hepatoma HepG2 Cells to High-LET Radiation,PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2010,37(1):79-84 |
Palavras-Chave | #THERAPEUTIC TARGET #CANCER CELLS #RADIOTHERAPY |
Tipo |
期刊论文 |