NADPH oxidase-mediated generation of reactive oxygen species: A new mechanism for X-ray-induced HeLa cell death
| Data(s) |
2008
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| Resumo |
Oxidative damage is an important mechanism in X-ray-induced cell death. Radiolysis of water molecules is a source of reactive oxygen species (ROS) that contribute to X-ray-induced cell death. In this study, we showed by ROS detection and a cell survival assay that NADPH oxidase has a very important role in X-ray-induced cell death. Under X-ray irradiation, the upregulation of the expression of NADPH oxidase membrane Subunit gp91(phox) was dose-dependent. Meanwhile, the cytoplasmic subunit p47(phox) was translocated to the cell membrane and localized with p22(phox) and gp91(phox) to form reactive NADPH oxidase. Our data Suggest, for the first time, that NADPH oxidase-mediated generation of ROS is an important contributor to X-ray-induced cell death. This suggests a new target for combined gene transfer and radiotherapy. National Natural Science Foundation of China 10675151 30870586 Postdoctoral Foundation of China 20070410232 Lanzhou Prajna Union Biology Technique Co., Ltd |
| Identificador | |
| Idioma(s) |
英语 |
| Fonte |
Liu, Q (Liu, Qing); He, XQ (He, Xiaoqing); Liu, YS (Liu, Yongsheng); Du, BB (Du, Bingbing); Wang, XY (Wang, Xiaoyan); Zhang, WS (Zhang, Weisheng); Jia, PF (Jia, Pengfei); Dong, JM (Dong, Jingmei); Ma, JX (Ma, Jianxiu); Wang, XH (Wang, Xiaohu); Li, S (Li, Sha); Zhang, H (Zhang, Hong) .NADPH oxidase-mediated generation of reactive oxygen species: A new mechanism for X-ray-induced HeLa cell death ,BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2008,377(3 ):775-779 |
| Palavras-Chave | #NADPH oxidase #New mechanism #X-ray irradiation #HeLa cell death |
| Tipo |
期刊论文 |
