长俊木瓜体细胞胚胎诱导的初步研究

以长俊木瓜为材料,研究了其体细胞胚胎诱导过程中各个环节的影响因素。结果表明:愈伤组织诱导的适宜外植体是叶片,培养基为MS+6-BA1.0 mg.L-1+2,4-D0.2 mg.L-1,黑暗培养;非胚性愈伤组织向胚性愈伤组织转化的适宜培养基是MS+6-BA1.0 mg.L-1+NAA1.0 mg.L-1;胚性愈伤组织的保持与增殖应在黑暗条件下进行,培养基为MS+6-BA1.0 mg.L-1+2,4-D0.2 mg.L-1;长俊木瓜叶片体胚的发生以加入ABA2.0 mg.L-1的MS培养基最为有利。

Chaenomeles speciosa(sweet) Nakai C.Lagenaria(Chang Jun) collected as material,the effects of various factors in the process of somatic embryogenesis from leaf blade were studied.The results indicated that the appropriate medium for callus induction was MS+6-BA1.0 mg·L-1+2,4-D 0.2 mg·L-1 with leaves as explants,darkness was useful;The appropriate medium for embryonic callus induction from callus was MS+6-BA1.0 mg·L-1+NAA1.0 mg·L-1;Embryonic callus had good multiplication ability in the medium MS with 6-BA1....