Low potential detection of glutamate based on the electrocatalytic oxidation of NADH at thionine/single-walled carbon nanotubes composite modified electrode
Data(s) |
2009
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Resumo |
A glutamate biosensor based on the electrocatalytic oxidation of reduced nicotinamide adenine dinucleotide (NADH), which was generated by the enzymatic reaction, was developed via employing a single-walled carbon nanotubes/thionine (Th-SWNTs) nanocomposite as a mediator and an enzyme immobilization matrix. The biosensor, which was fabricated by immobilizing glutamate dehydrogenase (GIDH) on the surface of Th-SWNTs, exhibited a rapid response (ca. 5 s), a low detection limit (0.1 mu M), a wide and useful linear range (0.5-400 mu M), high sensitivity (137.3 +/- 15.7) mu A mM(-1) cm(-2), higher biological affinity, as well as good stability and repeatability. In addition, the common interfering species, such as ascorbic acid, uric acid, and 4-acetamidophenol, did not cause any interference due to the use of a low operating potential (190 mV vs. NHE). The biosensor can be used to quantify the concentration of glutamate in the physiological level. The Th-SWNTs system represents a simple and effective approach to the integration of dehydrogenase and electrodes, which can provide analytical access to a large group of enzymes for wide range of bioelectrochemical applications including biosensors and biofuel cells. National Natural Science Foundation of China (20673057, 20773067);the Program for New Century Excellent Talents in University (NET-06-0508), and the "863" Project (2007AA05Z154) |
Identificador | |
Idioma(s) |
英语 |
Fonte |
Meng L (Meng, Ling);Wu P (Wu, Ping);Chen GX (Chen, Guoxiang);Cai CX (Cai, Chenxin);Sun YM (Sun, Yongming);Yuan ZH (Yuan, Zhenhong).Low potential detection of glutamate based on the electrocatalytic oxidation of NADH at thionine/single-walled carbon nanotubes composite modified electrode,BIOSENSORS & BIOELECTRONICS,2009,24(6):1751-1756 |
Palavras-Chave | #Biosensor #Dehydrogenase #Coenzyme #Glutamate #Carbon nanotubes #AMPEROMETRIC BIOSENSOR #DEHYDROGENASE #ENZYME #OXIDASE #GLUCOSE #BRAIN #PROBES #SENSOR #IONS |
Tipo |
期刊论文 |