Microcystin-RR-induced apoptosis in tobacco BY-2 cells


Autoria(s): Yin, Liyan; Huang, Jiaquan; Li, Wei; Liu, Yongding
Data(s)

01/08/2006

Resumo

Our previous studies showed that microcystin-RR could induce oxidative damage in plant cells as they do with animal cells. However, whether microcystin can induce plant cell apoptosis is still unknown. In this study, the morphological changes of tobacco BY-2 suspension cells exposed to microcystin-RR were observed under light microscopy and transmission electron microscopy, and apoptosis was clearly distinguished by intense perinuclear chromatin margination, condensation of nuclear chromatin after 6d exposure of 50 mg/L (about 50 mu M) microcystin-RR. We also found that microcystin-RR can induce tobacco cell apoptosis in a dose- and time-dependent manner with flow cytometry analysis. Our study provides the first evidence that microcystins can induce plant cell apoptosis. (c) 2006 Elsevier Ltd. All rights reserved.

Our previous studies showed that microcystin-RR could induce oxidative damage in plant cells as they do with animal cells. However, whether microcystin can induce plant cell apoptosis is still unknown. In this study, the morphological changes of tobacco BY-2 suspension cells exposed to microcystin-RR were observed under light microscopy and transmission electron microscopy, and apoptosis was clearly distinguished by intense perinuclear chromatin margination, condensation of nuclear chromatin after 6d exposure of 50 mg/L (about 50 mu M) microcystin-RR. We also found that microcystin-RR can induce tobacco cell apoptosis in a dose- and time-dependent manner with flow cytometry analysis. Our study provides the first evidence that microcystins can induce plant cell apoptosis. (c) 2006 Elsevier Ltd. All rights reserved.

Identificador

http://ir.ihb.ac.cn/handle/152342/8856

http://www.irgrid.ac.cn/handle/1471x/58941

Idioma(s)

英语

Fonte

Yin, Liyan; Huang, Jiaquan; Li, Wei; Liu, Yongding.Microcystin-RR-induced apoptosis in tobacco BY-2 cells,TOXICON,2006,48(2):204-210

Palavras-Chave #Pharmacology & Pharmacy; Toxicology #microcystin-RR #tobacco BY-2 cells #apoptosis #flow cytometry #transmission electron microscopy
Tipo

期刊论文