Sixteen polymorphic microsatellites in bighead carp (Aristichthys nobilis) and cross-amplification in silver carp (Hypophthalmichthys molitrix)


Autoria(s): Cheng, L.; Liu, L.; Yu, X.; Tong, J. .
Data(s)

01/05/2008

Resumo

A (GT)(n) enriched partial genomic library of bighead carp (Aristichthys nobilis) was constructed by employing the (fast isolation by AFLP of sequences containing repeats) FIASCO protocol. Sixteen loci exhibited polymorphism with two to seven alleles/locus (mean 3.263) in a test population and the observed heterozygosity ranging from 0.100 to 0.690 (mean 0.392). Eleven of the 16 bighead carp microsatellites were found to be also polymorphic in silver carp. These polymorphic loci should provide sufficient level of genetic diversity to evaluate population structure of bighead carp.

A (GT)(n) enriched partial genomic library of bighead carp (Aristichthys nobilis) was constructed by employing the (fast isolation by AFLP of sequences containing repeats) FIASCO protocol. Sixteen loci exhibited polymorphism with two to seven alleles/locus (mean 3.263) in a test population and the observed heterozygosity ranging from 0.100 to 0.690 (mean 0.392). Eleven of the 16 bighead carp microsatellites were found to be also polymorphic in silver carp. These polymorphic loci should provide sufficient level of genetic diversity to evaluate population structure of bighead carp.

Identificador

http://ir.ihb.ac.cn/handle/152342/8174

http://www.irgrid.ac.cn/handle/1471x/58600

Idioma(s)

英语

Fonte

Cheng, L.; Liu, L.; Yu, X.; Tong, J. ..Sixteen polymorphic microsatellites in bighead carp (Aristichthys nobilis) and cross-amplification in silver carp (Hypophthalmichthys molitrix),MOLECULAR ECOLOGY RESOURCES,2008,8(3):656-658

Palavras-Chave #Biochemistry & Molecular Biology; Ecology; Evolutionary Biology #aristichthys nobilis #cross-species amplification #genomic library #Hypophthalmichthys molitrix #microsatellite
Tipo

期刊论文