Chemical Constituents from the Leaves and Stems of Schisandra lancifolia

A new nortriterpenoid, 20-hydroxymicrandilactone D (1) and a novel lignan glycoside, lancilignanside A (2) were isolated from leaves and stems of Schisandra lancifolia, together with three known nortriterpenoids (3-5) and nine known phenolics (6-14). The structures of new compounds 1 and 2 were determined by detailed analysis of their 1D and 2D NMR spectra, and chemical evidences. In addition, compounds 1-2, 6-7, and 9-11 showed anti-human immunodeficiency virus (HIV)-1 activities with 50% effective concentration (EC50) in the range of 3.0-99.0 mu g/ml. Compound 12 was not bioactive in this assay with EC50 more than 200 mu g/ml.

This project was supported financially by the NSFC (No. 30830115 and 20802082), the projects from the Chinese Academy of Sciences ("Xibuzhiguang" to W.-L.X, KSCX1-YW-R-24, KSCX2-YW-R-185), 973 Program (No. 2009CB522300 and 2009CB940900), the Yong Academic and Technical Leader Rising Foundation of Yunnan Province (2006PY01-47) Key Scientific and Technology Projects of China (2009ZX09501-029). We would like to acknowledge MRC AIDS Research Project (UK) and NIH AIDS Research and Reference Reagent Program (USA) for providing cell lines and virus.