Cloning of novel bombesin precursor cDNAs from skin of Bombina maxima


Autoria(s): Shen, JH; Liu, SB; Zhang, YX; Jin, Y; Lee, WH; Zhang, Y
Data(s)

2005

Resumo

Amphibian skin is a rich resource of bioactive peptides like proline-rich bombesin from frog Bombina maxima. A novel cDNA clone encoding a precursor protein that comprises proline-rich bombesin and a novel peptide, designated as bombestatin, was isolated from a skin cDNA library of B. maxima. The predicted primary structure of the novel peptide is WEVLLNVALIRLELLSCRSSKDQDQKESCGMHSW, in which two cysteines form a disulfide bond. A BLAST search of databases did not detect sequences with significant similarity. Bombestatin possesses dose-dependent contractile activity on rat stomach strips. The differences between cDNAs encoding PR-bombesin plus bombestatin and PR-bombesin alone are due to fragment insertions located in 3'-coding region and 3'-untranslational region, respectively. (c) 2005 Elsevier B.V. All rights reserved.

Identificador

http://159.226.149.42/handle/152453/2663

http://www.irgrid.ac.cn/handle/1471x/46707

Direitos

Cloning of novel bombesin precursor cDNAs from skin of Bombina maxima

Fonte

Shen, JH; Liu, SB; Zhang, YX; Jin, Y; Lee, WH; Zhang, Y.Cloning of novel bombesin precursor cDNAs from skin of Bombina maxima,132,102-106,(SCI-E):

Palavras-Chave #Endocrinology & Metabolism; Physiology
Tipo

期刊论文