Identification and cloning of a trypsin inhibitor from skin secretions of Chinese red-belly toad Bombina maxima


Autoria(s): Lai, R; Liu, H; Lee, WH; Zhang, Y
Data(s)

2002

Resumo

A novel trypsin inhibitor was identified and purified from skin secretions of Chinese red-belly toad Bombina maxima. The partial N-terminal 29 amino acid residues of the peptide, named BMTI, were determined by automated Edman degradation. This allowed the cloning of a full-length cDNA encoding BMTI from a cDNA library prepared from the toad skin. The deduced complete amino acid sequence of BMTI indicates that mature BMTI is composed of 60 amino acids. A FASTA search in the databanks revealed that BMTI exhibits 81.7% sequence identity with BSTI, a trypsin/thrombin inhibitor from European toad Bombina bombina skin secretions. Sequence differences between BMTI and BSTI were due to 11 substitutions at positions 2, 9, 25, 27, 36-37, 39, 41-42, 50 and 56. BMTI potently inhibited trypsin with a K-i value of 0.06 muM, similar to that of BSTI. However, unlike BSTI, which also inhibited thrombin with a K-i value of 1 muM, no inhibitory effect of BMTI on thrombin was observed under the assay conditions. (C) 2002 Elsevier Science Inc. All rights reserved.

Identificador

http://159.226.149.42/handle/152453/2471

http://www.irgrid.ac.cn/handle/1471x/46652

Direitos

Identification and cloning of a trypsin inhibitor from skin secretions of Chinese red-belly toad Bombina maxima

Fonte

Lai, R; Liu, H; Lee, WH; Zhang, Y.Identification and cloning of a trypsin inhibitor from skin secretions of Chinese red-belly toad Bombina maxima,131,47-53,(SCI-E):

Palavras-Chave #Biochemistry & Molecular Biology; Zoology
Tipo

期刊论文