A cocktail of thermally stable, chemically synthesized capture agents for the efficient detection of anti-gp41 antibodies from human sera and techniques


Autoria(s): Pfeilsticker, Jessica A.
Data(s)

2014

Resumo

This thesis reports on a method to improve in vitro diagnostic assays that detect immune response, with specific application to HIV-1. The inherent polyclonal diversity of the humoral immune response was addressed by using sequential in situ click chemistry to develop a cocktail of peptide-based capture agents, the components of which were raised against different, representative anti-HIV antibodies that bind to a conserved epitope of the HIV-1 envelope protein gp41. The cocktail was used to detect anti-HIV-1 antibodies from a panel of sera collected from HIV-positive patients, with improved signal-to-noise ratio relative to the gold standard commercial recombinant protein antigen. The capture agents were stable when stored as a powder for two months at temperatures close to 60°C.

Formato

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Identificador

http://thesis.library.caltech.edu/8395/61/Thesis.pdf

http://thesis.library.caltech.edu/8395/1/CoverPage.pdf

http://thesis.library.caltech.edu/8395/2/Chapter%201.pdf

http://thesis.library.caltech.edu/8395/3/Chapter%202.pdf

http://thesis.library.caltech.edu/8395/4/Chapter%203.pdf

http://thesis.library.caltech.edu/8395/5/Chapter%204.pdf

http://thesis.library.caltech.edu/8395/28/Appendix%20A.pdf

http://thesis.library.caltech.edu/8395/33/Appendix%20B.pdf

http://thesis.library.caltech.edu/8395/39/Appendix%20C.pdf

http://thesis.library.caltech.edu/8395/49/Appendix%20D.pdf

http://thesis.library.caltech.edu/8395/54/Appendix%20E.pdf

Pfeilsticker, Jessica A. (2014) A cocktail of thermally stable, chemically synthesized capture agents for the efficient detection of anti-gp41 antibodies from human sera and techniques. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechTHESIS:05262014-170142267 <http://resolver.caltech.edu/CaltechTHESIS:05262014-170142267>

Relação

http://resolver.caltech.edu/CaltechTHESIS:05262014-170142267

http://thesis.library.caltech.edu/8395/

Tipo

Thesis

NonPeerReviewed