Expression and activity profiles of DPP IV/CD26 and NEP/CD10 glycoproteins in the human renal cancer are tumor-type dependent


Autoria(s): Varona Gutiérrez, Adolfo; Blanco Criado, Lorena; Pérez Urcelai, Itxaro; Gil Goicouría, Francisco Javier; Irazusta Astiazaran, Jon; López Fernández de Villaverde, José Ignacio; Candenas, M. Luz; Pinto, Francisco M.; Larrinaga Embeita, Gorka
Data(s)

01/04/2014

01/04/2014

01/05/2010

Resumo

Background: Cell-surface glycoproteins play critical roles in cell-to-cell recognition, signal transduction and regulation, thus being crucial in cell proliferation and cancer etiogenesis and development. DPP IV and NEP are ubiquitous glycopeptidases closely linked to tumor pathogenesis and development, and they are used as markers in some cancers. In the present study, the activity and protein and mRNA expression of these glycoproteins were analysed in a subset of clear-cell (CCRCC) and chromophobe (ChRCC) renal cell carcinomas, and in renal oncocytomas (RO). Methods: Peptidase activities were measured by conventional enzymatic assays with fluorogen-derived substrates. Gene expression was quantitatively determined by qRT-PCR and membrane-bound protein expression and distribution analysis was performed by specific immunostaining. Results: The activity of both glycoproteins was sharply decreased in the three histological types of renal tumors. Protein and mRNA expression was strongly downregulated in tumors from distal nephron (ChRCC and RO). Moreover, soluble DPP IV activity positively correlated with the aggressiveness of CCRCCs (higher activities in high grade tumors). Conclusions: These results support the pivotal role for DPP IV and NEP in the malignant transformation pathways and point to these peptidases as potential diagnostic markers.

Identificador

Bmc Cancer 10 : (2010) // Article n. 193

1471-2407

http://hdl.handle.net/10810/11897

10.1186/1471-2407-10-193

Idioma(s)

eng

Publicador

BioMed Central

Relação

http://www.biomedcentral.com/1471-2407/10/193

Direitos

© 2010 Varona et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

info:eu-repo/semantics/openAccess

Palavras-Chave #dipeptidyl peptidase IV #real time PCR #cell carcinoma #differential diagnosis #neutral endopeptidase #tachykinin receptors #extracellular matrix; #CD26 #CD10 #progression
Tipo

info:eu-repo/semantics/article