Recognition of Membrane-Bound Fusion-Peptide/MPER Complexes by the HIV-1 Neutralizing 2F5 Antibody : Implications for Anti-2F5 Immunogenicity
Data(s) |
19/02/2013
19/02/2013
21/12/2012
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Resumo |
13 p. The membrane proximal external region (MPER) of the fusogenic HIV-1 glycoprotein-41 harbors the epitope sequence recognized by 2F5, a broadly neutralizing antibody isolated from an infected individual. Structural mimicry of the conserved MPER 2F5 epitope constitutes a pursued goal in the field of anti-HIV vaccine development. It has been proposed that 2F5 epitope folding into its native state is attained in the vicinity of the membrane interface and might involve interactions with other viral structures. Here we present results indicating that oligomeric complexes established between MPER and the conserved amino-terminal fusion peptide (FP) can partition into lipid vesicles and be specifically bound by the 2F5 antibody at their surfaces. Cryo-transmission electron microscopy of liposomes doped with MPER:FP peptide mixtures provided the structural grounds for complex recognition by antibody at lipid bilayer surfaces. Supporting the immunogenicity of the membrane-bound complex, these MPER:FP peptide-vesicle formulations could trigger cross-reactive anti-MPER antibodies in rabbits. Thus, our observations suggest that contacts with N-terminal regions of gp41 may stabilize the 2F5 epitope as a membrane-surface antigen. |
Identificador |
PLoS ONE 7(12) : (2012) // e52740. 1932-6203 http://hdl.handle.net/10810/9465 10.1371/journal.pone.0052740 |
Idioma(s) |
eng |
Publicador |
Public Library of Science |
Relação |
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0052740 |
Direitos |
© 2012 Huarte et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. info:eu-repo/semantics/openAccess |
Palavras-Chave | #immunodeficiency-virus type-1 #proximal external region #large unilamellar vesicles #human monoclonal-antibody #complementarity-determining region #vaccine design #envelope glycoprotein #GP41 ectodomain #conformational constraints #anti-HIV-1 antibodies |
Tipo |
info:eu-repo/semantics/article |