Limited-view light sheet fluorescence microscopy for three dimensional volume imaging


Autoria(s): Rasmi, CK; Mohan, Kavya; Madhangi, M; Rajan, K; Nongthomba, U; Mondal, Partha P
Data(s)

2016

Resumo

We propose and demonstrate a limited-view light sheet microscopy (LV-LSM) for three dimensional (3D) volume imaging. Realizing that longer and frequent image acquisition results in significant photo-bleaching, we have taken limited angular views (18 views) of the macroscopic specimen and integrated with maximum likelihood (ML) technique for reconstructing high quality 3D volume images. Existing variants of light-sheet microscopy require both rotation and translation with a total of approximately 10-fold more views to render a 3D volume image. Comparatively, LV-LSM technique reduces data acquisition time and consequently minimizes light-exposure by many-folds. Since ML is a post-processing technique and highly parallelizable, this does not cost precious imaging time. Results show noise-free and high contrast volume images when compared to the state-of-the-art selective plane illumination microscopy. (C) 2015 AIP Publishing LLC.

Formato

application/pdf

Identificador

http://eprints.iisc.ernet.in/53285/1/App_Phy_Let_107-26_263701_2015.pdf

Rasmi, CK and Mohan, Kavya and Madhangi, M and Rajan, K and Nongthomba, U and Mondal, Partha P (2016) Limited-view light sheet fluorescence microscopy for three dimensional volume imaging. In: APPLIED PHYSICS LETTERS, 107 (26).

Publicador

AMER INST PHYSICS

Relação

http://dx.doi.org/10.1063/1.4938536

http://eprints.iisc.ernet.in/53285/

Palavras-Chave #Molecular Reproduction, Development & Genetics (formed by the merger of DBGL and CRBME) #Instrumentation and Applied Physics (Formally ISU) #Physics
Tipo

Journal Article

PeerReviewed