Optical microscopy methods for understanding learning and memory
Data(s) |
2013
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Resumo |
Structural dynamics of dendritic spines is one of the key correlative measures of synaptic plasticity for encoding short-term and long-term memory. Optical studies of structural changes in brain tissue using confocal microscopy face difficulties of scattering. This results in low signal-to-noise ratio and thus limiting the imaging depth to few tens of microns. Multiphoton microscopy (MpM) overcomes this limitation by using low-energy photons to cause localized excitation and achieve high resolution in all three dimensions. Multiple low-energy photons with longer wavelengths minimize scattering and allow access to deeper brain regions at several hundred microns. In this article, we provide a basic understanding of the physical phenomena that give MpM an edge over conventional microscopy. Further, we highlight a few of the key studies in the field of learning and memory which would not have been possible without the advent of MpM. |
Formato |
application/pdf |
Identificador |
http://eprints.iisc.ernet.in/48380/1/Cur_Sci_105-11_1537_2013.pdf Singh, Aditya and Kumar, Suraj and Singh, Vikram Pal and Balaji, J (2013) Optical microscopy methods for understanding learning and memory. In: CURRENT SCIENCE, 105 (11). pp. 1537-1548. |
Publicador |
INDIAN ACAD SCIENCES |
Relação |
http://www.currentscience.ac.in/Volumes/105/11/1537.pdf http://eprints.iisc.ernet.in/48380/ |
Palavras-Chave | #Centre for Neuroscience |
Tipo |
Journal Article PeerReviewed |