Optical microscopy methods for understanding learning and memory


Autoria(s): Singh, Aditya; Kumar, Suraj; Singh, Vikram Pal; Balaji, J
Data(s)

2013

Resumo

Structural dynamics of dendritic spines is one of the key correlative measures of synaptic plasticity for encoding short-term and long-term memory. Optical studies of structural changes in brain tissue using confocal microscopy face difficulties of scattering. This results in low signal-to-noise ratio and thus limiting the imaging depth to few tens of microns. Multiphoton microscopy (MpM) overcomes this limitation by using low-energy photons to cause localized excitation and achieve high resolution in all three dimensions. Multiple low-energy photons with longer wavelengths minimize scattering and allow access to deeper brain regions at several hundred microns. In this article, we provide a basic understanding of the physical phenomena that give MpM an edge over conventional microscopy. Further, we highlight a few of the key studies in the field of learning and memory which would not have been possible without the advent of MpM.

Formato

application/pdf

Identificador

http://eprints.iisc.ernet.in/48380/1/Cur_Sci_105-11_1537_2013.pdf

Singh, Aditya and Kumar, Suraj and Singh, Vikram Pal and Balaji, J (2013) Optical microscopy methods for understanding learning and memory. In: CURRENT SCIENCE, 105 (11). pp. 1537-1548.

Publicador

INDIAN ACAD SCIENCES

Relação

http://www.currentscience.ac.in/Volumes/105/11/1537.pdf

http://eprints.iisc.ernet.in/48380/

Palavras-Chave #Centre for Neuroscience
Tipo

Journal Article

PeerReviewed