High resolution methyl selective C-13-NMR of proteins in solution and solid state
Data(s) |
01/09/2012
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Resumo |
New C-13-detected NMR experiments have been devised for molecules in solution and solid state, which provide chemical shift correlations of methyl groups with high resolution, selectivity and sensitivity. The experiments achieve selective methyl detection by exploiting the one bond J-coupling between the C-13-methyl nucleus and its directly attached C-13 spin in a molecule. In proteins such correlations edit the C-13-resonances of different methyl containing residues into distinct spectral regions yielding a high resolution spectrum. This has a range of applications as exemplified for different systems such as large proteins, intrinsically disordered polypeptides and proteins with a paramagnetic centre. |
Formato |
application/pdf |
Identificador |
http://eprints.iisc.ernet.in/45136/1/Jou_of_Bio.pdf Jaipuria, Garima and Lobo, Nitin Prakash and Shet, Divya and Atreya, Hanudatta S (2012) High resolution methyl selective C-13-NMR of proteins in solution and solid state. In: Journal of Biomolecular NMR, 54 (1). pp. 33-42. |
Publicador |
Springer |
Relação |
http://dx.doi.org/10.1007/s10858-012-9647-0 http://eprints.iisc.ernet.in/45136/ |
Palavras-Chave | #NMR Research Centre (Formerly SIF) #Solid State & Structural Chemistry Unit #Physics |
Tipo |
Journal Article PeerReviewed |