A new type II restriction endonuclease, OfoI from nonheterocystous cyanobacterium Oscillatoria foreaui
Data(s) |
25/07/2003
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Resumo |
Although restriction enzymes are widely distributed in nature, many bacterial genera are yet to be explored for the presence of this important class of enzymes. We have purified and characterized a new type II restriction endonuclease, OfoI from a nonheterocyst cyanobacterium Oscillatoria foreaui. The recognition sequence has been determined by primer extension analysis. The purified enzyme OfoI recognizes and cleaves the palindromic hexanucleotide 5'-Cdown arrowYCGRG-3', generating 5'-protruding ends. |
Formato |
application/pdf |
Identificador |
http://eprints.iisc.ernet.in/39753/1/A_new_type_II_restriction.pdf Saravanan, Matheshwaran and Elango, Kathirvel and Chandrashekaran, Siddamadappa and Anand, Narayanaswamy and Nagaraja, Valakunja (2003) A new type II restriction endonuclease, OfoI from nonheterocystous cyanobacterium Oscillatoria foreaui. In: Current Science (Bangalore), 85 (2). pp. 188-191. |
Publicador |
Indian Academy of Sciences |
Relação |
http://www.ias.ac.in/currsci/jul252003/contents.htm http://eprints.iisc.ernet.in/39753/ |
Palavras-Chave | #Microbiology & Cell Biology |
Tipo |
Journal Article PeerReviewed |