Preferential condensation of SAR-DNA by histone H1 and its SPKK containing octapeptide repeat motif


Autoria(s): Khadake, Jyoti R; Rao, MRS
Data(s)

03/01/1997

Resumo

Linker histone H1 binds preferentially the scaffold associated region (SAR) DNA elements that contain characteristic oligo dA . dT tracts. In the present study, we have compared the condensation brought about by histone H1 of a SAR DNA fragment in the histone spacer region of Drosophila melanogaster with that of a random DNA (pBR322 EcoRI-SalI) fragment by circular dichroism spectroscopy. The condensation of the SAR DNA fragment by histone H1 is 3-4-fold higher than that of the random DNA fragment. A 16-mer peptide, ATPKKSTKKTPKKAKK, the sequence that is present in the C-terminus of histone H1d, which has recently been shown to possess DIVA and chromatin condensing properties, also condenses the SAR DNA fragment preferentially in a highly cooperative manner. We have proposed a model for the dynamics of chromatin structure involving histone H1-SAR DNA interaction through SPKK containing peptide motifs and its competition by AT-hook peptides present in the nonhistone chromosomal proteins like HMG-I and HMG-Y.

Formato

application/pdf

Identificador

http://eprints.iisc.ernet.in/38364/1/Preferential_condensation_o.pdf

Khadake, Jyoti R and Rao, MRS (1997) Preferential condensation of SAR-DNA by histone H1 and its SPKK containing octapeptide repeat motif. In: FEBS Letters, 400 (2). pp. 193-196.

Publicador

Elsevier Science

Relação

http://dx.doi.org/10.1016/S0014-5793(96)01393-2

http://eprints.iisc.ernet.in/38364/

Palavras-Chave #Biochemistry
Tipo

Journal Article

PeerReviewed