Production of biosurfactant “Biosur-Pm” by Pseudomonas maltophila CSV89: characterization and role in hydrocarbon uptake
Data(s) |
01/06/1995
|
---|---|
Resumo |
Pseudomonas maltophilia CSV89, a soil bacterium, produces an extracellular biosurfactant, ''Biosur-Pm''. The partially purified product is nondialyzable and chemically composed of 50% protein and 12-15% sugar, which indicates the complex nature of Biosur-Pm. It reduces the surface tension of water from 73 to 53 x 10(-3) N m(-1) and has a critical micellar concentration of 80 mg/l. Compared to aliphatic hydrocarbons, Biosur-Pm shows good activity against aromatic hydrocarbons. The emulsion formed is stable and does not require any metal ions for emulsification. The kinetics of Biosur-Pm production suggest that its synthesis isa growth-associated and pH-dependent process. At pH 7.0, cells produced more Biosur-Pm with less cell surface hydrophobicity. At pH 8.0, however, the cells produced less Biosur-Pm with more cell surface hydrophobicity and showed a twofold higher affinity for aromatic hydrocarbons compared to the cells grown at pH 7.0. The Biosur-Pm showed a pH-dependent release, stimulated growth of the producer strain on mineral salts medium with 1-naphthoic acid when added externally, and facilitated the conversion of salicylate to catechol. All these results suggest that Biosur-Pm is probably a cell-wall component and helps in hydrocarbon assimilation/uptake. |
Formato |
application/pdf |
Identificador |
http://eprints.iisc.ernet.in/38155/1/Production_of_biosurfactant.pdf Phale, Prashant S and Savithri, Handanahal Subbarao and Rao, Appaji N and Vaidyanathan, Chelakara S (1995) Production of biosurfactant “Biosur-Pm” by Pseudomonas maltophila CSV89: characterization and role in hydrocarbon uptake. In: Archives of Microbiology, 163 (6). pp. 424-431. |
Publicador |
Springer |
Relação |
http://www.springerlink.com/content/g24v3885t1475146/ http://eprints.iisc.ernet.in/38155/ |
Palavras-Chave | #Biochemistry |
Tipo |
Journal Article NonPeerReviewed |