Spatial gradients of calcium in the slug of Dictyostelium discoideum
Data(s) |
10/02/1995
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Resumo |
Starved amoebae of D. discoideum aggregate and give rise to a long and thin multicellular structure called the slug. The cells within the slug eventually differentiate according to a simple anterior/posterior dichotomy. This motivates a search for gradients of putative morphogens along its axis. Calcium may be one such morphogen. On the basis of observations made by using the calcium-sensitive fluorescent dyes fura-2 and chlortetracyline, we report that there are spatial gradients in cytoplasmic and sequestered calcium in the slug. Anteriorly located and genetically defined prestalk cells (ecmA/pstA, ecmB/pstAB) contain significantly higher levels of calcium than the prespore cells in the posterior. However, the proportion of 'calcium-rich' cells in the slug is greater than that of the subset of prestalk cells defined by the expression of the ecmA or ecmB genes. |
Formato |
application/pdf |
Identificador |
http://eprints.iisc.ernet.in/37912/1/Spatial.pdf Azhar, M and Saran, Shweta and Nanjundiah, Vidyanand (1995) Spatial gradients of calcium in the slug of Dictyostelium discoideum. In: Current Science (Bangalore), 68 (3). pp. 337-342. |
Publicador |
Indian Academy of Sciences |
Relação |
http://www.ias.ac.in/j_archive/currsci/68/vol68contents.html http://eprints.iisc.ernet.in/37912/ |
Palavras-Chave | #Others |
Tipo |
Editorials/Short Communications PeerReviewed |