Characterization of an in vitro transcription system from rinderpest virus
Data(s) |
01/05/1995
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Resumo |
An in vitro transcription system for rinderpest virus (RPV) is described. Ribonucleoprotein complexes isolated from RPV-infected Vero cells, human lung carcinoma cells, or detergent-disrupted purified virions synthesized authentic RPV mRNAs for the N, P, M, F and H genes as identified by dot blot hybridization analysis with individual cDNA clones. The relative abundance of the mRNAs synthesized in vitro decreased from the 3? end of the genome to the 5? end, very similar to that observed with measles virus transcription in vitro. The transcription by purified virions was stimulated three-fold by the addition of infected human lung carcinoma cell lysate, demonstrating the involvement of host factor(s) in mRNA synthesis. |
Formato |
application/pdf |
Identificador |
http://eprints.iisc.ernet.in/37228/1/Vitro.pdf Ghosh, Arundhati and Joshi, Vishwas D and Shaila, MS (1995) Characterization of an in vitro transcription system from rinderpest virus. In: International Symposium on Morbillivirus Infections, JUN 12-13, 1994, HANNOVER VET SCH, HANNOVER, GERMANY. |
Publicador |
Elsevier Science |
Relação |
http://dx.doi.org/10.1016/0378-1135(95)00009-Y http://eprints.iisc.ernet.in/37228/ |
Palavras-Chave | #Microbiology & Cell Biology #Others |
Tipo |
Conference Paper PeerReviewed |