Structural proteins of mycobacteriophage I3: cloning, expression and sequence analysis of a gene encoding a 70-kDa structural protein


Autoria(s): Ramesh, Gundmi R; Gopinathan, Karumathil P
Data(s)

27/05/1994

Resumo

The structural proteins of mycobacteriophage I3 have been analysed by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis (SDS-PAGE), radioiodination and immunoblotting. Based on their abundance the 34- and 70-kDa bands appeared to represent the major structural proteins. Successful cloning and expression of the 70-kDa protein-encoding gene of phage I3 in Escherichia coli and its complete nucleotide sequence determination have been accomplished, A second (partial) open reading frame following the stop codon for the 70-kDa protein was also identified within the cloned fragment. The deduced amino-acid sequence of the 70-kDa protein and the codon usage patterns indicated the preponderance of codons, as predicted from the high G+C content of the genomic DNA of phage I3.

Formato

application/pdf

Identificador

http://eprints.iisc.ernet.in/36836/1/Structural_proteins.pdf

Ramesh, Gundmi R and Gopinathan, Karumathil P (1994) Structural proteins of mycobacteriophage I3: cloning, expression and sequence analysis of a gene encoding a 70-kDa structural protein. In: Gene, 143 (1). pp. 95-100.

Publicador

Elsevier science

Relação

http://dx.doi.org/10.1016/0378-1119(94)90611-4

http://eprints.iisc.ernet.in/36836/

Palavras-Chave #Microbiology & Cell Biology #Molecular Reproduction, Development & Genetics (formed by the merger of DBGL and CRBME)
Tipo

Editorials/Short Communications

PeerReviewed