Intracellular free calcium level and its response to cAMP stimulation in developing Dictyostelium cells transformed with jellyfish apoaequorin CDNA
Data(s) |
03/01/1994
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Resumo |
A new method is described for measuring intracellular free calcium concentrations, [(Ca2+)(i)], in the cells of Dictyostelium discoideum transformed with apoaequorin cDNA of the jellyfish, Aequorea victoria. Aequorin, a calcium-specific indicator, was regenerated in vivo from apoaequorin produced in the cells by incubation with coelenterazine. The results showed that [(Ca2+)(i)] in developing cells markedly increases at the aggregation stage and again at the culmination stage after a temporary drop at the migration stage. Except for the vegetative stage, the cells al all stages of development exhibit a sharp transient increase in [(Ca2+)(i)] upon stimulation with a cAMP (50 nM) pulse, high responses being observed at the migration and culmination stages. Separated prestalk cells of migrating slugs contain more than twice as much [(Ca2+)(i)] and show three times as large a response to cAMP stimulation as prespore cells. |
Formato |
application/pdf |
Identificador |
http://eprints.iisc.ernet.in/36558/1/INTERMETALLIC__t12.pdf Saran, S and Nakao, H and Tasaka, M and Iida, H and Tsuji, FI and Nanjundiah, V and Takeuchi, I (1994) Intracellular free calcium level and its response to cAMP stimulation in developing Dictyostelium cells transformed with jellyfish apoaequorin CDNA. In: FEBS letters, 337 (1). pp. 43-47. |
Publicador |
Elsevier Science |
Relação |
http://dx.doi.org/10.1016/0014-5793(94)80626-8 http://eprints.iisc.ernet.in/36558/ |
Palavras-Chave | #Others |
Tipo |
Journal Article PeerReviewed |