High level stable expression of rat brain type IIA sodium channel ?-subunit in CHO cells
Data(s) |
10/08/1994
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Resumo |
The neuronal sodium channels are responsible for the rising phase of action potential and are composed of three subunits, of which the alpha-subunit has been shown to be adequate for most of its functional properties. We have stably expressed the rat brain type IIA sodium channel alpha-subunit in CHO cell tine using a CMV promoter-based vector. The expression was confirmed by detecting a 6.5 kb RNA corresponding to sodium channel alpha-subunit using Northern hybridization. The cells stably expressing the alpha-subunit, yield isolated sodium currents of amplitudes greater than 4nA when studied in whole-cell configuration of the patch-clamp technique. The sodium currents are characterized by activation and inactivation properties similar to neuronal sodium channels, and are blocked by the voltage gated sodium channel blocker tetrodotoxin (TTX). |
Formato |
application/pdf |
Identificador |
http://eprints.iisc.ernet.in/36532/1/High.pdf Sarkar, SN and Sikdar , SK (1994) High level stable expression of rat brain type IIA sodium channel ?-subunit in CHO cells. In: Current science (Bangalore), 67 (3). 196 -199 . |
Publicador |
Indian academy of sciences |
Relação |
http://www.ias.ac.in/j_archive/currsci/67/vol67contents.html http://eprints.iisc.ernet.in/36532/ |
Palavras-Chave | #Molecular Biophysics Unit |
Tipo |
Editorials/Short Communications PeerReviewed |